Schedule for week of July 28

Thanks to everyone who helped with lab cleanup!!
Jack

Cloning hsp27 promoter
Looking for ready-made constructs
Designing a 2nd approach to cloning hsp27 from Zfin sequences
Re-evaluate cloning of vtg1 promoter
Harassing people

June

Fish exposure (EE2 and C60)
Cyp1a1 and Ef-1 genes cloning

Emily

Go to JARTU twice daily to check on catfish
Weigh out salt daily for columnaris treatment
Treat catfish for columnaris infection daily [...]

Lab outcomes – week July 21

Mon

Bought webcam for general lab use; stored in top shelf of left cabinet
Raising larave solely on 18-hr brine shrimp test

Started with 300 embryos – 249 larvae hatched, 248 survived
Larvae switched to beakers last Sat (day 4); 18-hr hatched BS feeding started
Larvae not observed eating BS (D4-D6)
Received San Francisco Bay Brine Shrimp – made new cone [...]

Schedule for week of July 21

Jack
Screwing up things for 40 hours
June
CYP1a1 and EF-1 gene setup for qRT PCR analysis
Designing experiments of nanoparticles and endocrine disruptors
Emily

Go to JARTU twice daily to check on catfish
Monitor catfish for columnaris infection and treat tanks if necessary
Purchase remaining supplies for catfish experiment from Kroger and Lowes
Make contaminant-spiked food for catfish experiment
Develop tissue sampling procedure for [...]

Lab outcomes – week July 14

Mon

Water quality test – pH & ammonia
All big tanks: pH between 6.5-7.5; ammonia level (w/o calculation, obtained from amm. disc) 0.1-0.2
Jan/Feb tank: pH of 4.7; ammonia level 0.7 – did 50% water change
Water change on all medium tanks

Tues

Spawning: ~1200 embryos
Saved 300 to raise on BS (testing)
20% water change on all big tanks

Thurs

Spawning: ~1500 embryos
~350 for [...]

Schedule for week of July 14

Jack
Out from Tues-Sun
June
Cloning EF-1 and CYP1a1 (if possible)
Design of experiments for C60/EE2 exposure, PAH, and dietary exposure of nanoparticles
Emily
Monday-Friday: AM work at JARTU; PM work at SERF
Wed: 1pm Wilhelm lab meeting
Sat-Sun: feed fish at JARTU
Activities: feed/maintain catfish at JARTU, prepare food for catfish experiment, revise experimental design, work on manuscript
Ping
Mon             Measure pH, ammonia etc.
Tues/Thurs  Spawning
Wed              [...]