Mon
- Bought webcam for general lab use; stored in top shelf of left cabinet
- Raising larave solely on 18-hr brine shrimp test
- Started with 300 embryos – 249 larvae hatched, 248 survived
- Larvae switched to beakers last Sat (day 4); 18-hr hatched BS feeding started
- Larvae not observed eating BS (D4-D6)
- Received San Francisco Bay Brine Shrimp – made new cone @ 3.30pm
- Continued hatching regular BS @ 18-hr
- Water change on all medium tanks
- Switched June 2008 juveniles to glass bowl
- Discarded 200 larvae saved for algae method – spirulina powder not ready
Tues
- Water quality test – pH & ammonia
All big tanks: pH between 5.06-7.37; ammonia level (w/o calculation, obtained from amm. disc) 0.1-0.3 - Siphoned debris, did 20% water change and cleaned filters on Male T (pH 6.27) & Female T (pH 5.06) tank
- Excellent spawning: 16/18 tanks, ~3500 embryos
- ~250 for microinjection, saving the rest
- Larvae labeled as 072208
- Larvae raising
- SF BS hatched well – fed to larvae
- Feeding behavior observed in some larvae; also saw distinct orange bellies (yay)
- Larvae count – 247
Wed
- Pipettes calibrated
- ~3250 embryos doing well; cleaned and changed water twice
- Larvae raising – current count 245
- Lab cleanup – changed table top covers, discarded unsued/old items, reorganized cabinet etc.
Thurs
- Spawning: 7/11 tanks, ~1700 embryos; 2 big females not releasing eggs
- Saving all eggs for Ping and June’s experiment next week
- Larvae labeled as 072408
- Larvae raising – switched larvae to 1.5L plastic tank; current count 243
- Lab cleanup – clearing glass vials
- Paramecia counting attempt using hemocytometer was not good – paramecia were hard to see
- Sent enquiry to Zfin
- Also tried with dissection scope – better view – ~50 paramecia/mL
Fri
- Ongoing experiment: PH#9 Part 1 started – running until Tues
- Lab cleanup – disposing/cleaning glass vials
- Larvae 072208 disposed
- Larvae 072408 used for PH#9 Part 2 & June’s experiment
Filed under: Lab outcomes
