Thanks to everyone who helped with lab cleanup!!
Jack
- Cloning hsp27 promoter
- Looking for ready-made constructs
- Designing a 2nd approach to cloning hsp27 from Zfin sequences
- Re-evaluate cloning of vtg1 promoter
- Harassing people
June
- Fish exposure (EE2 and C60)
- Cyp1a1 and Ef-1 genes cloning
Emily
- Go to JARTU twice daily to check on catfish
- Weigh out salt daily for columnaris treatment
- Treat catfish for columnaris infection daily with 0.5% salt treatment (~2h holding period)
- Make contaminant-spiked food for catfish experiment a few days before experiment (depends on start date, which is TBD)
- Develop tissue sampling procedure for catfish experiment (amount of tissue per sample, determine if pooling tissues is necessary)
- Continue revising manuscript
Ping
Mon – setup spawning tanks; water change; start PH#9 Part 2
Tues – collect embryos (saving for experiments & microinjection); water quality test; end PH#9 Part 1
Wed – setup spawning tanks
Thurs – collect embryos (saving some for microinjection)
Fri – end PH#9 Part 2
Also working on organizing order forms
Ann
- Catfish Treatment ~10hrs
- Paramecia ~2hrs
- Microcystis research (didn’t happen last week) ~3 hrs
- Change website pic and Title ~10 min
- Run concentrated microcystis on yeast bioreporter
- Dr. Wilhelm lab meeting
TOTAL ~15 hrs
Candace
Monday 3-5 (Setup; 2h)
Tuesday 9-1 (injections; 4h)
Wednesday 3-5 (setup; 2h)
Thursday 9-1 (injections; 4h)
Friday 12-1 (counting; 1h)
Saturday 12-1 (counting; 1h)
Sunday 12-1 (counting; 1h)
Total: 15h
Jessica
Filed under: Schedules
