Lab outcomes week Nov 24-28

Mon

Setup spawning tanks
All October larvae doing well
Dissection practice – June & Ping
Separated males (44) and females (64) in Jan/Feb batch
Microcystis & EE2 experiment started (Ann’s)

Tues

Spawning: ~500 embryos; discarded
No microinjection
Water change in medium tank and bowl

Wed

Dissection practice
Paramecia split
Separated males (64) & females (68) in March-July batch

Thurs

Thanksgiving holiday
Changed water for larvae

Fri

Thanksgiving holiday
Ending Ann’s experiment

Lab outcomes – week Nov 17-21

Mon

Setup spawning tanks
Larvae 102108 Day 27 & 102308 Day 25 – BS
102808 Day 20 – BS
103008 Day 18 – BS
All larvae changed to new 1L tank
111308 hatched (still in petri dish)
Put 111108 in beaker
Histology: Sectioning (DeOnna)
Contacted Scott about fish room temperature

Tues

Spawning: ~300 embryos; all kept and labeled as 111808
Microinjection ~130 embryos
Fish room overheated again
102108 Day [...]

Schedule – week of Nov 24

Jack
1.  Real Time PCR Vtg Type 1 Std – DNA and RNA.
2.  Real Time PCR B-actin on PH9 Samples
3.  Screen Vtg1 Promoter Clones – Got good colonies using new primer.
4.  Hopefully more Real Time
June
CYP1a1 RT-PCR condition setup and draft work
Emily
Ping
Mon – setup spawning
Tues – collect embryos
Wed – setup spawning
Thurs – collect embryos
Fri – water change; [...]

Schedule – week of Nov 17

Jack
1.  Vtg 1 promoter region
2.  Vtg Type 1 Std
3.  GST-pi Std
4.  Real Time
5.  Reverse transcription
6.  DNA stds for sybr green assay
June
CYP1a1-RT, meeting with Ted
Emily
Prelims
Ping
Mon – setup spawning
Tues – collect embryos
Wed – setup spawning
Thurs – collect embryos
Fri – water change; WQ test
*Monitor larvae growth
Ann
Paramecia ~4hours
Finish preparing microcystis~6hours
Finish weighing vials ~2hours
Enter data~2hours
TOTAL ~14hours

Candace
Jessica
Helping Jack
Rachel
Friday – [...]

Lab outcomes – Nov 10-14

Mon

Setup spawning tanks
Larvae 102108 Day 20 & 102308 Day 18 – BS; growing well
102808 Day 13 – BS; growing well
103008 Day 11 – BS; observed some orange bellies
Discarded JWP P#1 – mass dying in exposure beakers
Setup new JWP P#1 @ 50% of original concentration

Tues

Spawning: ~150 embryos; all kept and labeled as 111108
Microinjection ~50 embryos
Fish room [...]

Lab outcomes – Nov 3-7

Mon

Setup spawning tanks
Larvae 102108 Day 13 – a few died; other than that, all good;
Larvae 102308 Day 11 – good
10283108 Day 6 – good
All larvae fed sonicated food; BS added to 102108 and 102308

Tues

Spawning: ~600-800 embryos
All kept and labeled as 110408
No microinjection
Transferred 102108 fish from larvae box back into beaker
102108 Day 14 – BS only; [...]

Schedule – week of Nov 10

Jack
1.  Vtg 1 promoter region
2.  Vtg Type 1 Std
3.  GST-pi Std
4.  Real Time
5.  Reverse transcription
6.  DNA stds for sybr green assay
June
CYP1a1-RT, meeting with Ted
Emily
Prelims

Ping
Mon – setup spawning
Tues – collect embryos
Wed – setup spawning
Thurs – collect embryos
Fri – water change; WQ test
*Monitor larvae growth
Ann
Paramecia ~4hours
Finish preparing microcystis~6hours
Finish weighing vials ~2hours
Enter data~2hours
TOTAL ~14hours

Candace
Jessica
Helping Jack
Rachel
Friday – [...]