Posted on January 30, 2009 by tedhenry
Mon
C60 observation
Water change
RNA extraction comparison
Tues
C60 observation with cytoviva
Wed
Histology – Rachel D. training
Setup spawning tanks
Lab cleanup
Nov/Dec fish mixed
Thurs
Spawning: ~800 embryos; all discarded
No microinjection – Candace went to pull needles
Breakdown tanks; setup tanks
RNA extraction
C60 observation with cytoviva
Fri
Spawning: ~ 800 embryos; all discarded
No microinjection
Histology
Microscopy with John Dunlap
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Posted on January 30, 2009 by tedhenry
Jack
RNA extraction
June
Artemia experiment draft
Dietary experiment draft
CYP1a
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – microscopy; setup spawning; water change
Tues – meeting; collect embryos; breakdown/setup spawning
Wed – microscopy; collect embryos; breakdown spawning
Thurs – microscopy
Fri – microscopy; work on website
Ann
Mon
Centrifuge microcystis ~1hr
Check paramecia ~2hrs
Split paramecia ~2hrs
Tues
Ship catfish samples (?) ~1hr
Make BG-11 media ~1hr
Meeting ~ .5-1hr
Wed
Check paramecia ~2hr
Thurs
Help Candace ~.5hr
Microscope ~1hr
Centrifuge and [...]
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Posted on January 23, 2009 by tedhenry
Jack
Unknown yet
Wishing it would snow more
June
Cytoviva observation, CYP1a cloning, and experimental draft
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – cytoviva observation
Tues – cytoviva observation; water change
Wed – cytoviva observation; setup spawning
Thurs – cytoviva observation; collect embryos; setup spawning
Fri – cytoviva observation; collect embryos
Ann
Monday
Microscope work ~1 hr
Centrifuging microcystis ~6hrs
Check Paramecia ~2hrs
Tuesday
Make BG-11 Media ~2hrs
split paramecia ~2hrs
Wed
Check Paramecia ~2hrs
Split microsystis [...]
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Posted on January 23, 2009 by tedhenry
Mon
MLK Day
Tues
Water change in medium tanks & big tanks
Wed
RNA extraction
Setup spawning tanks
Jack & Ping met with Jill
Thurs
Spawning: ~200 embryos; all discarded
No microinjection – Candace was sick
Breakdown tanks; setup tanks
RNA extraction
Cytotiva observation
Paramecia checked
Fri
Spawning: ~ 500 embryos; labeled 012309
Microinjection – ~250 embryos
Histology
Cytoviva observation
Rachel D. doing histology with DeOnna and Rachel J.
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Posted on January 20, 2009 by tedhenry
Jack
June
Emily
Ping
Mon – MLK Day
Tues – RNA extraction; water change
Wed – setup spawning
Thurs – collect embryos; setup spawning
Fri – collect embryos
Ann
Candace
Total = 14 HOURS
1/20 Tuesday = 1
+ sonicate stock solution 1 (for thursday)
1/21 Wednesday = 2
+ sonicate stock solution 2 (for friday if needed)
+ make gels
+ set-up
1/22 Thursday = 5
+ mix & sonicate DiI solutions
+ inject [...]
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Posted on January 20, 2009 by tedhenry
Mon
Setup spawning tanks
Changed water in glass bowls and medium tanks
Tidied up fish room and dry lab
Tues
Spawning: ~400 embryos; labeled 011309
Microinjection – ~300 embryos
Water change in big tanks
Wed
Setup spawning tanks
Checked paramecia
011309 embryo survival – ~60/100
Water change in big tank
RNA extraction
Thurs
Spawning: ~500 embryos; labeled 011509
Microinjection – ~250 embryos
Water change in big tank
RNA extraction
Fri
Water change in big tanks
011309 [...]
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