Lab outcomes Feb 23-27

Mon

Larvae 021009  – 3 beakers (~70 larave/beaker) – fed sonicated food first then brine shrimp
Larvae 021109 – 2 beakers (~140 larvae/beaker) – fed brine shrimp all the way
Larvae 021809 – 1 beaker (103) – fed sonicated food and brine shrimp at the same time
Young juveniles from January doing very well
Microscopy – artemia experiment
Setup spawning tanks

Tues

Spawning: [...]

Schedule – week of March 2

Jack
Getting the YFP construct going
June
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change in medium tanks; brine shrimp acid treatment experiment; larvae care
Tues – collect embryos; break down/setup spawning; brine shrimp acid treatment experiment
Wed – collect embryos; break down / setup Ann’s spawning tanks; reproduction exp.; brine shrimp acid treatment experiment
Thurs – reproduction exp.; brine [...]

Schedule – week of Feb 23

Jack
Getting the YFP construct going
June
C60 measurement in brine shrimp
Elimination of C60 from brine shrimp
Zebrafish EE2 exposure via diet
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change in medium tanks; setup brine shrimp-C60 elimination experiement; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – end elimination test; [...]

Lab outcomes Feb 16-20

Mon

Water change – medium tank
Larvae care – 3 beakers (~90 larave/beaker) from 021009, 2 beakers from 021109 (~140 larvae/beaker)
Made new sonicated larvae food – for 021209; discarded old one
Fed 021109 brine shrimp
Tidied up fish room to make way for Ann’s experiment
Paramecia checked; 20 boxes made from last week’s petri dishes
Setup June’s artemia test; end on [...]

Schedule – week of Feb 16

Jack
Doesn’t know yet
June
Brine shrimp experiments
Draft for EE2 dietary exposure to fish
Dietary experiment design
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change in medium & big tanks; setup brine shrimp test; take care of larvae
Tues – collect embryos; break down/setup spawning; water change in big tanks
Wed – collect embryos; break down spawning/setup spawning
Thurs – end brine [...]

Lab outcomes – Feb 9-13

Mon

Water change – medium tanks
Siphoned big tanks; 30% water change
Paramecia split – new flasks made, petri dishes made
Paramecia count: 110 flasks, 20 petri dishes
Setup June’s artemia test

Tues

Spawning: ~400 embryos; labeled as 021009
No microinjection – Candace is sick
Breakdown/setup tanks
Jill training with Ping (breakdown tanks, make/collect brine shrimp)
Histology practice – Rachel D.

Wed

Spawning: ~500 embryos; labeled as 021109
No [...]

Lab outcomes Feb 2-6

Mon

C60 observation
Setup spawning tanks
Catfish primer arrived

Tues

Spawning: ~200 embryos; kept for Candace
No microinjection
Breakdown/setup tanks
RT-PCR
Jill training with Jack
Ping taught Jill to make fish water and breakdown spawning tanks
C60 observation
Histology practice – Rachel D.

Wed

Spawning: ~500 embryos; labeled as 020409
Microinjection
Breakdown tanks
Water quality test
Paramecia flask count: 102

Thurs

pH test
Paramecia checked
Jack & Emily are sick

Fri

No histology – DeOnna and Rachel are both [...]

Schedule – week of Feb 9

Jack

June
Artemia experiment draft
Dietary experiment draft
CYP1a

Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change
Tues – collect embryos; breakdown/setup spawning
Wed – collect embryos; breakdown spawning
Thurs -
Fri – work on website; water quality tests
Ann
Mon
Check Paramecia~2hr
Microscope ~0.5hr
Centrifuge microcystis ~5hr
Tues
Microscope~1hr
Sex fish~2hr
Practice measuring fish ~1hr
Split paramecia ~2hr
Wed
Check paramecia~2hr
Microscope~1hr
Split microcystis ~0.5hr
Thurs
Sex fish~2hr
Dechorionate eggs ~1hr
pPractice egg counting ~1hr
Fri
Check paramecia~2hr
Microscope~1hr
Total ~24hrs
Candace
Rachel
Tuesday 11-12pm Work with [...]