Lab outcomes – March 23-27

Mon

Setup spawning tanks
All larvae in glass bowls
Water changes in medium tanks
60% water change in Mar-Jul big tank
30% water change in Male & Female spawning tanks
Rinsed all filters; changed new filters for new fish tanks – discarded old filters
Re-setup brine shrimp experiment
Flat, 96-well plates arrived

Tues

Spawning: ~300 embryos; kept for Candace; labeled 032409
No microinjection
Breakdown/setup tanks
Re-setup brine shrimp [...]

Schedules – week of March 30

Jack
?????

June
Emily
Ontology analysis
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – end elimination test; break down spawning with Ann
Fri – work on website; water quality tests
Ann
Monday
Mon
Check paramecia ~30min
Make BG-11 media ~1hr
Take pics and clean eggs ~1hr
Tues
Centrifuge microcystis ~7hr
Practice [...]

Schedules – week of March 23

Jack
Battling “lovely stomach thing”

June
Manuscript and experimental draft works
Emily
Ontology analysis
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – end elimination test; break down spawning with Ann
Fri – work on website; water quality tests
Ann
Monday
Clean eggs /pics~1hr
Check paramecia and pick [...]

Lab outcomes March 16-20

Mon

Setup spawning tanks
Larvae care

Tues

Spawning: ~300 embryos; kept for Candace; labeled 031709
No microinjection
Breakdown/setup tanks
Larvae care

Wed

Spawning: ~350 embryos; labeled 031809
Microinjection ~180 embryos
Breakdown and setup spawning tanks for reproduction experiment
Major cleaning and water change of experimental tanks
Larvae 021009 & 021109 in glass bowls
Larvae 021809 & 022509 (previously on paramecia) in plastic boxes – all on BS
Water change in [...]

Schedules – week of March 16

Jack
Specific Aims
cyp1a1 screening etc
June
ACS abstract
EE2 dietary exposure
C60 measurement in brine shrimp
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – end elimination test; break down spawning with Ann
Fri – work on website; water quality tests
Ann
Monday
Clean [...]

Lab outcomes March 9-13

Mon

Larvae 021009  – 2 boxes (~80 larave/beaker) – on brine shrimp now
Larvae 021109 – glass bowl (~200 larvae/beaker) – on brine shrimp now
Larvae 021809 – 1 beaker (~74) – on brine shrimp
Larvae 022509 – 3 beakers (~98/beaker) – already on brine shrimp; growing well
Setup spawning tanks
Diet exposure experiment
Water change in medium tanks & glass bowl
Setup [...]

Schedules – week of March 9

Jack
Specific Aims
cyp1a1 screening etc
June
ACS abstract
EE2 dietary exposure
C60 measurement in brine shrimp
Emily
M-F 9am-5pm
Pathway analysis
Reproduction experiment
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – end elimination test; break down spawning with Ann
Fri – work on website; water quality tests
Ann
Monday
Clean [...]

Lab outcomes March 2-8

Mon

Larvae 021009  – 2 boxes (~85 larave/beaker) – on brine shrimp now
Larvae 021109 – 2 boxes (~130 larvae/beaker) – on brine shrimp now
Larvae 021809 – 1 beaker (~82) -  sonicated food and brine shrimp at the same time
Larvae 022509 – 3 beakers (100/beaker) – paramecia only; paramecia + brine shrimp; paramecia + sonicated food
Young juveniles [...]