Posted on April 24, 2009 by tedhenry
Mon
Setup spawning tanks
Switched all juveniles from glass bowls to medium tanks
M & F spawning tanks – 60% water change
New M & F tanks – 60% water change
Tues
Spawning: ~300 embryos; kept for Candace; labeled 042109
No microinjection
Breakdown/setup tanks
Centrifuged larvae with Ann
Experiment with brine shrimp
Jill did RNA extraction
Wed
Spawning: ~1000 embryos; labeled 042209
Microinjection ~300 embryos
Breakdown and setup spawning tanks [...]
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Posted on April 24, 2009 by tedhenry
Jack
June
Emily
Ontology analysis
Ping
Regular work – fish colony maintenance & spawning
Experiment – brine shrimp (Mon-Wed)
Ann
Exam week
Candace
Rachel & DeOnna & Rachel D.
Exam week
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Posted on April 17, 2009 by tedhenry
Jack
?????
June
Emily
Ontology analysis
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – break down spawning with Ann
Fri – work on website; water quality tests
Ann
Mon
Check paramecia ~30min
Make BG-11 media ~1hr
Take pics and clean eggs ~1hr
Tues
Centrifuge microcystis ~7hr
Practice weighing and [...]
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Posted on April 17, 2009 by tedhenry
Mon
Setup spawning tanks
Water changes in all medium tanks & glass bowls
Setup brine shrimp for preliminary dietary exposure experiment
Setup the above mentioned experiment
Tues
Spawning: ~300 embryos; kept for Candace; labeled 041409
No microinjection
Breakdown/setup tanks
Centrifuged larvae with Ann
Start preliminary dietary exposure experiment
Histology – DeOnna
Wed
Spawning: ~600 embryos; labeled 041509
Microinjection ~250 embryos
Breakdown and setup spawning tanks for reproduction experiment
Major cleaning and [...]
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Posted on April 10, 2009 by tedhenry
Mon
Setup spawning tanks
Water changes in medium tanks & glass bowls
C60 centrifugation failed – Dietary preliminary experiment could not be started
Tues
Spawning: ~500 embryos; kept for Candace; labeled 040709
No microinjection
Breakdown/setup tanks
Centrifuged larvae with Ann
Wed
Spawning: ~420 embryos; labeled 040809
No microinjection
Breakdown and setup spawning tanks for reproduction experiment
Major cleaning and water change of experimental tanks
Histology – Rachel, DeOnna, Rachel [...]
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Posted on April 10, 2009 by tedhenry
Jack
?????
June
Emily
Ontology analysis
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – break down spawning with Ann
Fri – work on website; water quality tests
Ann
Mon
Check paramecia ~30min
Make BG-11 media ~1hr
Take pics and clean eggs ~1hr
Tues
Centrifuge microcystis ~7hr
Practice weighing and [...]
Filed under: Schedules | Leave a Comment »
Posted on April 3, 2009 by tedhenry
Jack
?????
June
Emily
Ontology analysis
Ping
Mon – setup spawning; water change in medium tanks; take care of larvae
Tues – collect embryos; break down/setup spawning
Wed – collect embryos; break down spawning/setup spawning
Thurs – break down spawning with Ann
Fri – work on website; water quality tests
Ann
Mon
Check paramecia ~30min
Make BG-11 media ~1hr
Take pics and clean [...]
Filed under: Schedules | Leave a Comment »
Posted on April 3, 2009 by tedhenry
Mon
Setup spawning tanks
Water changes in medium tanks & glass bowls
Emptied 2 big tanks for new fish – refilled with new water
Decapsulation failed; setup 2nd attempt
Sexed new fish
Tues
Spawning: ~300 embryos; kept for Candace; labeled 033109
No microinjection
Breakdown/setup tanks
Decapsulation 2nd attempt failed; asked DeOnna for guidance
Sexed new fish
Microcystis experiment: practiced weighing and measuring fish
Wed
Spawning: ~570 embryos; labeled 040109
No [...]
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