Mon
Memorial Day
Tues
Setup 24 spawning tanks – Tues & Wed spawning
Washed and rinsed filters of spawning M & F tanks
Oct 08 & Jan 09 tank – 60% water change; filters rinsed
Water change in all medium tanks and glass bowls
Redo brine shrimp experiment
Larvae care
Wed
Spawning: ~1000 embryos; used in PH#20
Breakdown/setup tanks
No microinjection
@ JARTU with Ann
Cont. BS exp.
Microcystis culturing [...]
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Mon
Setup spawning tanks
Water change in all medium tanks & glass bowl
Tues
Spawning: ~400 embryos; used in PH#20
No microinjection
Breakdown/setup tanks
RT-PCR with June
Wed
Spawning: ~150 embryos; used in PH#20
No microinjection
Checked paramecia
Breakdown/setup tanks
RT-PCR with June
Thurs
Spawning: ~170 embryos; used in PH#20
Worked at JARTU with Ann
Fri
Made paramecia boxes
Meeting
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Mon
Setup spawning tanks
Water change in all medium tanks & glass bowl
Tues
Spawning: ~900 embryos; used in PH#20
No microinjection
Breakdown/setup tanks
Wed
Spawning: ~400 embryos; used in PH#20
No microinjection
30% water change in M & F spawning tanks, Jan-July 08 tank and Oct 08 & Jan 09 tank
50% water change in New M & F tanks
Checked paramecia
Thurs
Split paramecia
Made petri dishes and [...]
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Mon
Setup spawning tanks
Water change in all medium tanks & glass bowl
Experiment: Measuring ingested C60 amount by cultured BS
Tues
Spawning: ~600 embryos; labeled 050509
No microinjection
Breakdown/setup tanks
Brine shrimp experiment
Wed
Spawning: ~400 embryos; labeled 040609
No microinjection
Breakdown /setup tanks for PH#20
Brine shrimp experiment
Thurs
Water quality test
Breakdown tanks – obtained 6 fish for Day 32 and Day 4
Brine shrimp experiment
Checked paramecia
Fri
Brine shrimp experiment
Rinsed [...]
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Mon
Setup spawning tanks
Water change in all medium tanks
Brine shrimp counting experiment
Larvae care
Tues
Spawning: ~1700 embryos; labeled 042809
No microinjection
Breakdown/setup tanks
Centrifuged last batch of larvae – exposure experiment officially ended
Brine shrimp counting experiment
Larvae care – day 6; on brine shrimp
Wed
Spawning: ~700 embryos; labeled 042909
No microinjection
Bleached all tanks used in exposure experiment
Brine shrimp counting experiment
Larvae care – day 7
Thurs
Water [...]
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Jack
June
Emily
Ontology analysis
Ping
Regular work – fish colony maintenance & spawning
Ann
Exam week
Candace
Rachel & DeOnna & Rachel D.
Exam week
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