Lab outcomes – June 15-19

Mon

Water changes – all medium tanks
30% water changes – New M, New F and Feb 10 & 11 tanks
60% water change – Oct/Jan tank
10% water changes – M & F spawning tanks
Setup spawning tanks
Larvae care

Tues

Spawning: ~180 embryos; all used for microinjection
Microinjection
Squeezed females
RNA extraction
Breakdown/setup spawning tanks
Larvae care

Wed

Spawning: ~800 embryos; kept for Candace
Breakdown/setup tanks
Squeezed females
Checked paramecia

Thurs

Lab cleanup

Fri

Split [...]

Lab outcomes – June 8-12

Mon

Setup spawning tanks
Larvae care
Setup June’s experiment

Tues

Spawning: ~500 embryos; all used in PH#20
No microinjection
Water change in all medium tanks and glass bowls
Breakdown/setup spawning tanks
Larvae care
Started June’s experiment
RNA extraction – PH#20

Wed

Spawning: 100 embryos
Microinjection ~100 embryos
Breakdown tanks
PH#20 officially ended

Thurs

Checked paramecia
Larvae care

Fri

Larvae care
Water quality test
Prepare to end June’s experiment on Saturday

Water Quality Test

Oct 08 & Jan 09 tank
pH [...]

Lab outcomes June 1-5

Mon

100% water change in New F, New M and Jan-July tank
All three tanks washed, filters rinsed
Switched Jan-July fish into medium tanks and put 2.10.09 & 2.11.09 fish into big tank
Setup spawning tanks
Larvae care

Tues

Spawning: ~500 embryos; all used in PH#20
No microinjection
Water change in all medium tanks and glass bowls
Checked paramecia
Breakdown/setup spawning tanks
Larvae care

Wed

Spawning: 0 embryos
Breakdown/setup tanks
No [...]