Lab outcomes – June 15-19

Mon Water changes – all medium tanks 30% water changes – New M, New F and Feb 10 & 11 tanks 60% water change – Oct/Jan tank 10% water changes – M & F spawning tanks Setup spawning tanks Larvae care Tues Spawning: ~180 embryos; all used for microinjection Microinjection Squeezed females RNA extraction Breakdown/setup [...]

Lab outcomes – June 8-12

Mon Setup spawning tanks Larvae care Setup June’s experiment Tues Spawning: ~500 embryos; all used in PH#20 No microinjection Water change in all medium tanks and glass bowls Breakdown/setup spawning tanks Larvae care Started June’s experiment RNA extraction – PH#20 Wed Spawning: 100 embryos Microinjection ~100 embryos Breakdown tanks PH#20 officially ended Thurs Checked paramecia [...]

Lab outcomes June 1-5

Mon 100% water change in New F, New M and Jan-July tank All three tanks washed, filters rinsed Switched Jan-July fish into medium tanks and put 2.10.09 & 2.11.09 fish into big tank Setup spawning tanks Larvae care Tues Spawning: ~500 embryos; all used in PH#20 No microinjection Water change in all medium tanks and [...]