"The UT Zebrafish Research Facility" Directed by Dr. Ted Henry

Lab outcomes – June 15-19

tedhenry — Sat, 20 Jun 2009 03:04:01 +0000

Mon

Water changes – all medium tanks
30% water changes – New M, New F and Feb 10 & 11 tanks
60% water change – Oct/Jan tank
10% water changes – M & F spawning tanks
Setup spawning tanks
Larvae care

Tues

Spawning: ~180 embryos; all used for microinjection
Microinjection
Squeezed females
RNA extraction
Breakdown/setup spawning tanks
Larvae care

Wed

Spawning: ~800 embryos; kept for Candace
Breakdown/setup tanks
Squeezed females
Checked paramecia

Thurs

Lab cleanup

Fri

Split paramecia – 10 new flasks made
Water quality test

Water Quality Test

Jan-July tank
pH 4.75
Amm. 0.4
Nitrite 0.04

Oct 08 & Jan 09 tank
pH 6.94
Amm. 0.1
Nitrite 0

Male spawning tank
pH 7.43
Amm. 0.1 x 0.015 = 0.0015
Nitrite 0

Female spawning tank
pH 7.37
Amm. 0.1 x 0.015 = 0.0015
Nitrite 0

New fish female tank
pH 4.52
Amm. 0.3
Nitrite 0

New fish male tank
pH 5.39
Amm. 0.4
Nitrite 0

Lab outcomes – June 8-12

tedhenry — Sat, 13 Jun 2009 03:47:30 +0000

Mon

Setup spawning tanks
Larvae care
Setup June’s experiment

Tues

Spawning: ~500 embryos; all used in PH#20
No microinjection
Water change in all medium tanks and glass bowls
Breakdown/setup spawning tanks
Larvae care
Started June’s experiment
RNA extraction – PH#20

Wed

Spawning: 100 embryos
Microinjection ~100 embryos
Breakdown tanks
PH#20 officially ended

Thurs

Checked paramecia
Larvae care

Fri

Larvae care
Water quality test
Prepare to end June’s experiment on Saturday

Water Quality Test

Oct 08 & Jan 09 tank
pH 4.10
Amm. 0.6
Nitrite 0

Feb 09 Juv tank
pH 6.95
Amm. 0.2
Nitrite 0.02 x 3.33 = 0.0666

Male spawning tank
pH 7.40
Amm. 0.1 x 0.015 = 0.0015
Nitrite 0

Female spawning tank
pH 7.40
Amm. 0.1 x 0.015 = 0.0015
Nitrite 0

New fish female tank
pH 6.63
Amm. 0.3
Nitrite (Ran out of nitrite reagent)

New fish male tank
pH 6.73
Amm. 0.2
Nitrite 0.03 x 3.33 = 0.0999

Lab outcomes June 1-5

tedhenry — Fri, 05 Jun 2009 18:03:58 +0000

Mon

100% water change in New F, New M and Jan-July tank
All three tanks washed, filters rinsed
Switched Jan-July fish into medium tanks and put 2.10.09 & 2.11.09 fish into big tank
Setup spawning tanks
Larvae care

Tues

Spawning: ~500 embryos; all used in PH#20
No microinjection
Water change in all medium tanks and glass bowls
Checked paramecia
Breakdown/setup spawning tanks
Larvae care

Wed

Spawning: 0 embryos
Breakdown/setup tanks
No microinjection
Paramecia split: 14 new flasks made, 5 new boxes made
RNA extraction – PH#20

Thurs

Spawning: 0 embryos
No microinjection
Breakdown tanks
RNA extraction (whole fish) – June’s preliminary experiment
Larvae care

Fri

Larvae care
RT-PCR (from yesterday’s RNA extraction results)
Meeting with June

Sat

PH#20 Day 4 dissection

Lab outcomes – May 25-29

tedhenry — Sat, 30 May 2009 03:05:16 +0000

Mon

Memorial Day

Tues

Setup 24 spawning tanks – Tues & Wed spawning
Washed and rinsed filters of spawning M & F tanks
Oct 08 & Jan 09 tank – 60% water change; filters rinsed
Water change in all medium tanks and glass bowls
Redo brine shrimp experiment
Larvae care

Wed

Spawning: ~1000 embryos; used in PH#20
Breakdown/setup tanks
No microinjection
@ JARTU with Ann
Cont. BS exp.
Microcystis culturing with Ann
Larvae care

Thurs

Spawning: 0 embryos –> no microinjection
Cont. BS exp.
Microcystis culturing with Ann
Meeting with June
Larvae care

Fri

Checked paramecia
Made paramecia boxes
Water quality test
Larvae care
Split microcystis
Cont. BS exp.

Water Quality Test

Jan-July tank
pH 4.72
Amm. 3.0
Nitrite 0

Oct 08 & Jan 09 tank
pH 6.26
Amm. 0.2
Nitrite 0

Male spawning tank
pH 7.50
Amm. 0
Nitrite 0

Female spawning tank
pH 7.56
Amm. 0
Nitrite 0

New fish female tank
pH 4.26
Amm. >3.0
Nitrite 0

New fish male tank
pH 4.34
Amm. >3.0
Nitrite 0

Lab outcomes – May 18-22

tedhenry — Sat, 23 May 2009 03:58:22 +0000

Mon

Setup spawning tanks
Water change in all medium tanks & glass bowl

Tues

Spawning: ~400 embryos; used in PH#20
No microinjection
Breakdown/setup tanks
RT-PCR with June

Wed

Spawning: ~150 embryos; used in PH#20
No microinjection
Checked paramecia
Breakdown/setup tanks
RT-PCR with June

Thurs

Spawning: ~170 embryos; used in PH#20
Worked at JARTU with Ann

Fri

Made paramecia boxes
Meeting

Lab outcomes – May 11-15

tedhenry — Sat, 16 May 2009 03:17:00 +0000

Mon

Setup spawning tanks
Water change in all medium tanks & glass bowl

Tues

Spawning: ~900 embryos; used in PH#20
No microinjection
Breakdown/setup tanks

Wed

Spawning: ~400 embryos; used in PH#20
No microinjection
30% water change in M & F spawning tanks, Jan-July 08 tank and Oct 08 & Jan 09 tank
50% water change in New M & F tanks
Checked paramecia

Thurs

Split paramecia
Made petri dishes and boxes

Fri

Rinsed out used scintillation vials from last year\’s catfish project

Water Quality Test

Jan-July tank
pH 7.21
Amm. 0.2 x 0.0096 = 0.00192
Nitrite 0.03 x 3.33 = 0.0999

Oct 08 & Jan 09 tank
pH 7.32
Amm. 0.1 x 0.0123 = 0.00123
Nitrite 0

Male spawning tank
pH 7.13
Amm. 0.1 x 0.0078 = 0.00078
Nitrite 0

Female spawning tank
pH 7.12
Amm. 0.1 x 0.0078 = 0.00078
Nitrite 0

New fish female tank
pH 5.26
Amm. 0.3
Nitrite 0

New fish male tank
pH 4.70
Amm. 0.4
Nitrite 0

Lab outcomes – May 4-8

tedhenry — Sat, 09 May 2009 03:46:14 +0000

Mon

Setup spawning tanks
Water change in all medium tanks & glass bowl
Experiment: Measuring ingested C60 amount by cultured BS

Tues

Spawning: ~600 embryos; labeled 050509
No microinjection
Breakdown/setup tanks
Brine shrimp experiment

Wed

Spawning: ~400 embryos; labeled 040609
No microinjection
Breakdown /setup tanks for PH#20
Brine shrimp experiment

Thurs

Water quality test
Breakdown tanks – obtained 6 fish for Day 32 and Day 4
Brine shrimp experiment
Checked paramecia

Fri

Brine shrimp experiment
Rinsed out used scintillation vials from last year\’s catfish project

Water Quality Test

Jan-July tank
pH 7.21
Amm. 0.2 x 0.0096 = 0.00192
Nitrite 0.03 x 3.33 = 0.0999

Oct 08 & Jan 09 tank
pH 7.32
Amm. 0.1 x 0.0123 = 0.00123
Nitrite 0

Male spawning tank
pH 7.13
Amm. 0.1 x 0.0078 = 0.00078
Nitrite 0

Female spawning tank
pH 7.12
Amm. 0.1 x 0.0078 = 0.00078
Nitrite 0

New fish female tank
pH 5.26
Amm. 0.3
Nitrite 0

New fish male tank
pH 4.70
Amm. 0.4
Nitrite 0

Lab outcomes – April 27-May 1

tedhenry — Sat, 02 May 2009 03:09:35 +0000

Mon

Setup spawning tanks
Water change in all medium tanks
Brine shrimp counting experiment
Larvae care

Tues

Spawning: ~1700 embryos; labeled 042809
No microinjection
Breakdown/setup tanks
Centrifuged last batch of larvae – exposure experiment officially ended
Brine shrimp counting experiment
Larvae care – day 6; on brine shrimp

Wed

Spawning: ~700 embryos; labeled 042909
No microinjection
Bleached all tanks used in exposure experiment
Brine shrimp counting experiment
Larvae care – day 7

Thurs

Water quality test
M & F spawning tanks – 50% water change
March-July tank (renamed as Jan-July tank) – 100% water change; tank washed; filter rinsed; combine with Jan/Feb fish
Jan/Feb tank (renamed as Juv tank) – 100% water change; tank washed; filter rinsed; put Oct 08 & Jan 09 juveniles
Rinsed out used scintillation vials from last year’s cat fish project
Larvae care

Fri

Rinsed out used scintillation vials from last year’s cat fish project
New F & M tanks – 70% water change; filter rinsed

Water Quality Test

Jan/Feb tank
pH 7.81
Amm. 0.1 x 0.0370 = 0.00370
Nitrite 0.02 x 3.33 = 0.0666

Mar-July tank
pH 4.16
Amm. 0.8
Nitrite 003 x 3.33 = 0.0999

Male spawning tank
pH 6.70
Amm. 0.1
Nitrite 0

Female spawning tank
pH 6.90
Amm. 0.1
Nitrite 0

New fish female tank
pH 4.20
Amm. 0.5
Nitrite 0

New fish male tank
pH 4.39
Amm. 1.0
Nitrite 0

Schedules – May 4-8

tedhenry — Sat, 02 May 2009 03:09:32 +0000

Jack

June

Emily

Ontology analysis

Ping

Regular work – fish colony maintenance & spawning

Ann

Exam week

Candace

Rachel & DeOnna & Rachel D.

Exam week

Lab outcomes – April 20-24

tedhenry — Sat, 25 Apr 2009 03:34:32 +0000

Mon

Setup spawning tanks
Switched all juveniles from glass bowls to medium tanks
M & F spawning tanks – 60% water change
New M & F tanks – 60% water change

Tues

Spawning: ~300 embryos; kept for Candace; labeled 042109
No microinjection
Breakdown/setup tanks
Centrifuged larvae with Ann
Experiment with brine shrimp
Jill did RNA extraction

Wed

Spawning: ~1000 embryos; labeled 042209
Microinjection ~300 embryos
Breakdown and setup spawning tanks for reproduction experiment
Major cleaning and water change of experimental tanks

Thurs

Breakdown spawning tanks
End exposure experiment
Larvae care

Fri

Water quality test
Larvae care – growing up 200

Sat & Sun

Culture brine shrimp for 2 days – start experiment the following week
Fish colony maintenance

Water Quality Test

Jan/Feb tank
pH 6.13
Amm. 0.1
Nitrite 0

Mar-July tank
pH 4.83
Amm. 0.2
Nitrite 0

Male spawning tank
pH 7.00
Amm. 0.1 x 0.0060 = 0.00060
Nitrite 0

Female spawning tank
pH 7.08
Amm. 0.1 x 0.0078 = 0.00078
Nitrite 0

New fish female tank
pH 6.65
Amm. 0.1
Nitrite 0

New fish male tank
pH 6.65
Amm. 0.1
Nitrite 0.02